Fig. 4

MYH1G-AS facilitates myoblast proliferation but inhibits myogenic differentiation. A Relative MYH1G-AS expression with MYH1G-AS interference in CPMs. B Volcano plots of differentially expressed genes between the control group and MYH1G-AS interference. C Relative mRNA expressions of several differentially expressed genes after MYH1G-AS interference detected by RNA-seq. D−M Relative mRNA expressions of several cell cycle-inhibiting genes (D), EdU proliferation assay (E), proliferation rate of myoblasts (F), cell cycle analysis (G), CCK-8 assay (H), MyHC immunostaining (I), myotube area (J), differentiation index (K), myoblast fusion index (L), and relative mRNA (M) and protein (N) expression levels of myoblast differentiation marker genes with MYH1G-AS inhibition in vitro. In panel N, the numbers shown below the bands were folds of band intensities relative to the control. Band intensities were quantified by ImageJ and normalized to β-tubulin. Results are presented as mean ± SEM. In panels A, C, D, F−H, and J−M, statistical significance of differences between means was assessed using an independent sample t-test. (*P < 0.05; **P < 0.01)