Fig. 8

SMAD4 is required for the function of MYH1G-AS. A Relative SMAD4 expression after SMAD4 interference. B–P Relative SMAD4 expression (B), relative mRNA expression of several cell cycle-inhibiting genes (C), EdU proliferation assays (D), proliferation rate of myoblasts (E), cell cycle analysis (F), MyHC immunostaining (G), myotube area (H), differentiation index (I), myoblast fusion index (J), relative mRNA (K) and protein (L) expression levels of myoblast differentiation marker genes, relative mtDNA content (M), mitochondrial membrane potential (N), [ROS]i (O), and relative mRNA expressions of FBXO25 (P) induced by the listed nucleic acids in CPMs. In panel L, the numbers shown below the bands are folds of band intensities relative to the control. Band intensities were quantified by ImageJ and normalized to β-tubulin. Results are presented as mean ± SEM. In panels A–C, E, F, H–K, and M–P, the statistical significance of differences between means was assessed using an independent sample t-test. (*P < 0.05; **P < 0.01; NS, no significant difference)