Fig. 3

RHBDD2 was a direct target of miR-4739. A Predicted binding site between miR-4739 and RHBDD2 3′-UTR. B, C Luciferase activity for HeLa/DDP and SiHa/DDP cells cotransfected with WT-RHBDD2 or MUT-RHBDD2 and miR-4739 mimics or NC mimics. D Relative expression of RHBDD2 mRNA in HeLa/DDP and SiHa/DDP cells transfected with miR-4739 mimics or NC mimics were determined by quantitative real time PCR. **p < 0.01, ***p < 0.001, compared with NC mimics via Student’s t-test. E RHBDD2 protein levels in the transfected cells were evaluated by western blot analysis and GAPDH was used as the control. F The expression of RHBDD2 was determined via quantitative real-time PCR in DDP-resistant (n = 35) and DDP-sensitive tissues (n = 35) both from CC patients. G Spearman rank correlation analysis showed an inverse correlation between the expression levels of RHBDD2 and miR-4739 in DDP-resistant tissues from patients with