Fig. 4

Knockdown of RHBDD2 promoted the chemosensitivity of CC cells to DDP in vitro. HeLa/DDP and SiHa/DDP cells were transfected with si-RHBDD2 or si-NC for 48 h, and then subjected to treatment with or without 2 μg/ml DDP for 24 h. A The expression of RHBDD2 mRNA levels were determined in the above HeLa/DDP and SiHa/DDP cells. B, C The cell viability of the above HeLa/DDP and SiHa/DDP cells was determined using CCK-8 assay. D IC50 values of the above HeLa/DDP and SiHa/DDP cells were calculated and shown. E, F Cell apoptosis rate was determined by flow cytometry assay. G, H Transwell assay was applied to analyze cell migration ability of the above HeLa/DDP and SiHa/DDP cells. *p < 0.05, **p < 0.01, ***p < 0.001, compared with si-NC via Student’s t-test