Fig. 5

Overexpression of RHBDD2 reversed the regulatory of miR-4739 on DDP resistance in CC cells. HeLa/DDP cells were transfected with pcDNA3.1, pcDNA3.1–RHBDD2, or pcDNA3.1–RHBDD2 along with miR-4739 mimics. A The protein expression of RHBDD2 was detected by western blot analysis. B The cell viability of the transfected HeLa/DDP cells was determined using CCK-8 assay. C IC50 values of the transfected HeLa/DDP cells were calculated and shown. D Cell apoptosis rate was determined by flow cytometry assay. E Transwell assay was applied to analyze cell migration ability of the transfected HeLa/DDP cells. F The protein levels of RHBDD2, P-gP, MRP1, cleaved caspase-3, and E-cadherin were determined by western blot analysis. **p < 0.01, ***p < 0.001, compared with NC mimics + pcDNA3.1; #p < 0.05, ##p < 0.01, compared with miR-4739 mimics + pcDNA3.1