Fig. 9

SUMOylation sites in eIF5A are essential to drive proliferation of PANC-1 cells and to promote PANC-1 cells migration in vitro. A Western blot analysis of RhoA and Ras in PANC-1 cells transiently transfected with HA–eIF5A1–WT, HA–eIF5A1–3KR, HA–eIF5A1–5KR, or the empty vector pcDNA. B PANC-1 cells stably transfected with pcDNA, HA–eIF5A1–WT, HA–eIF5A1–3KR, or HA–eIF5A1–5KR were evaluated for cell growth. Graphs show the proliferation of PANC-1 cells after the indicated times of growth. Data represent the mean and error bars of three biological replicates. ANOVA **P < 0.01, ****P < 0.0001 compared with the cells overexpressing eIF5A–WT; ###P < 0.001, ####P < 0.0001 compared with the pcDNA transfected cells. C, Migration of PANC1 cells stably transfected with pcDNA, HA–eIF5A1–WT, HA–eIF5A1–3KR, or HA–eIF5A1–5KR was determined by transwell migration assay (left panel). Data represent the mean and error bars of three biological replicates. ANOVA *P < 0.05, **P < 0.01, ***P < 0.001 compared with the cells overexpressing eIF5A–WT; ##P < 0.01 compared with the pcDNA transfected cells. Right panel shows western blot analysis of the expression of HA–eIF5A–WT or the mutant proteins in the PANC-1 cells analyzed in B and C