Table 2 Yeast strains
From: SUMOylation modulates eIF5A activities in both yeast and pancreatic ductal adenocarcinoma cells
Strain | Genotype | Source |
|---|---|---|
PAY717 | MATa hyp2-3 | [49] |
PAY723 | MATa BNI1–HA | [49] |
PAY725 | MATa hyp2-1 BNI1–HA | [49] |
YTL710 | MATa LEU2::pAG425GPD–ccdB | This study |
YTL718 | PAY717 LEU2::pAG425GPD–ccdB | This study |
YTL720 | PAY717LEU2::pAG425GPD–eIF5a–WT–FTH | This study |
YTL722 | PAY717 LEU2::pAG425GPD–eIF5a–K50R–FTH | This study |
YTL724 | PAY717 LEU2::pAG425GPD–eIF5a–3KA–FTH | This study |
YTL726 | PAY723 LEU2::pAG425GPD–ccdB | This study |
YTL734 | PAY725 LEU2::pAG425GPD–ccdB | This study |
YTL736 | PAY725 LEU2::pAG425GPD–eIF5a–WT–FTH | This study |
YTL738 | PAY725 LEU2::pAG425GPD–eIF5a–K50R–FTH | This study |
YTL740 | PAY725 LEU2::pAG425GPD–eIF5a–3KA–FTH | This study |
YTL1393 | PAY725 LEU2::pAG425GPD–ccdB URA3::pRS416–PAB1–GFP | This study |
YTL1396 | PAY725 LEU2::pAG425GPD–eIF5a–WT–FTH URA3::pRS416–PAB1–GFP | This study |
YTL1399 | PAY725 LEU2::pAG425GPD–eIF5a–K50R–FTH URA3::pRS416–PAB1–GFP | This study |
YTL1404 | PAY725 LEU2::pAG425GPD–eIF5a–3KA–FTH URA3::pRS416–PAB1–GFP | This study |
YTL1823 | PAY725 LEU2::pAG425GPD–eIF5a–3KR–FTH | This study |
YTL1826 | PAY725 LEU2::pAG425GPD–eIF5a–5KR–FTH | This study |
YTL1829 | PAY717 LEU2::pAG425GPD–eIF5a–3KR–FTH | This study |
YTL1832 | PAY717 LEU2::pAG425GPD–eIF5a–5KR–FTH | This study |
YTL1857 | YTL710 LEU2::pAG425GPD–ccdbURA3::pRS416–PAB1–GFP | This study |
YTL1861 | PAY725 LEU2::pAG425GPD–eIF5a–5KR–FTH URA3::pRS416–PAB1–GFP | This study |