Fig. 7
Comparison of single-cell analysis before and after normalization. A UMAP plots representing batch effects from replicates before Harmony, B UMAP plots showing the correction of batch effects after Harmony. C Clustree plot for determining resolution with principal components (PCs). D Unified manifold approximation and projection clustering into 13 clusters. E Cells from human peripheral blood samples were annotated using CellMarker and singleR. F FeaturePlots showing the expression pattern of FBXO7, PGS1, BCS1L, LYRM7, MRPS31, and TIMMDC1 in peripheral blood mononuclear cells (PBMCs) from SCM and control groups. G Dot plot shows the expression levels of hub genes in each cell cluster. H The violin plot displays the gene expression of FBXO7 in each cell cluster. *p < 0.05, **p < 0.01, ***p < 0.001