Fig. 3

Pancreatic organoid-derived cells form a polarized epithelium when cultured on a permeable substrate. A Top panel: transverse section of an epithelial monolayer cultured on a Transwell insert (HE-stain). Scale bar: 25 µm. Bottom panel: the apical aspect of a confluent monolayer (bright-field microscopy). Scale bar: 100 µm. B Epithelial monolayers (top) and pancreatic tissue (bottom) were stained for SOX9. Nuclei are visualized using DAPI staining. Insets show SOX9 staining only in the area denoted by an asterisk. Scale bars: 50 µm. C–F Epithelial monolayers (top panels) and pancreatic tissue (bottom panels) were stained for KRT7, ECAD, ZO-1 or CFTR. Nuclei are visualized using DAPI staining. Scale bars: 50 µm. Fluorescence was visualized on a Stellaris 5 low incidence angle upright confocal microscope with a HC PL APO CS2 40x/1.30 oil objective (Leica Microsystems)