Fig. 6

Effect of pro-inflammatory cytokines on anion transport in pancreatic ductal organoid-derived epithelial monolayers. A Effect of combined IL-1β, IL-6, IFN-γ and TNF-α exposure (20 h) on the expression of genes involved in ductal anion secretion. B Representative experiment showing the effect of combined IL-1β, IL-6, IFN-γ and TNF-α exposure (20 h) on the forskolin-dependent, PPQ-102-sensitive, Isc response. C Forskolin-dependent Isc responses in cytokine-treated and control monolayers. The inset shows the change in Isc elicited by PPQ-102. D The effect of different cytokines and combinations thereof on the CFTR-mediated Isc response. Experiments were performed on cultures obtained from three animals. Connecting lines indicate paired experiments performed in parallel, on monolayers cultured from the same organoid line. Statistical analysis: paired t-test (A, C), ANOVA (D). ^aP = 0.02, ^bP = 0.007, ^cP < 0.001, compared to untreated