Table 1 Primer sequences used for RT-PCR
From: Pro-inflammatory cytokines stimulate CFTR-dependent anion secretion in pancreatic ductal epithelium
Gene | Accession number | Forward | Reverse |
|---|---|---|---|
ANO1 (TMEM16A) | XM_021082670.1 | CACCTTCATGGAGCACTGGA | GGATGGTCCTCCTCCTCCTC |
ANO6 (TMEM16F) | XM_005655713.3 | CGCCAGGCAGAACTTGAGTA | CACGTGAGTACACTGTGCCT |
CFTR | NM_001104950.1 | TCATGCCGGGCACCATTAAA | CGCTTTGATGACACTCCTGTATCTA |
SLC4A2 (AE2) | XM_021078861.1 | CCGGTTGATGATGTCGAGGT | CAGCTGGTCCCTGTGAAGTC |
SLC4A4 (NBCe1) | NM_001030533.1 | TCTTTGCGACAGGTCGTGTA | CTTCCACGCTCTCAGTGGAC |
SLC9A1 (NHE1) | NM_001007103.1 | CCCCCAAGGACCAGTTCATC | AGGTCTACCAGAGGCCGAAT |
SLC12A2 (NKCC1) | XM_003123899.5 | GGATGGCAAGACTCCAACTCA | CCTCCATCATCAAAAAGCCACC |
SLC26A4 (Pendrin) | XM_003357511.4 | TGGTGGGATCTGTTGTTCTGA | ACTCTTGCTGCGTCTCTAGC |
SLC26A6 (PAT1) | NM_001012298.2 | ATGGGGCTGTCAGAAGCG | GCCTCCTCAGCTCCATTGTT |
YWHAZ | NM_001315726.1 | GGAGCCCGTAGGTCATCTTG | ATTCTCGAGCCATCTGCTGT |