Fig.1

Identification and characterization of circRNF216 in CRC tissues. A, B Heatmap and Volcano plots generated from RNA-seq analysis shows differential expression of circRNAs. C qRT-PCR was performed on total RNA extracted from 4 pairs of CRC and normal tissues. D, E circRNF216 expression in 40 paired CRC and corresponding adjacent non-tumorous tissue samples was detected by qRT-PCR. F Survival curve was plotted by using Kaplan–Meier estimates. G qRT-PCR analysis of the relative circRNF216 levels in CRC cell lines. H The divergent primers detected circRNF216 in cDNA but not in gDNA. GAPDH was used as a positive control. I HCT116 cells were treated with actinomycin D to determine the half-life of circRNF216 and its linear transcript. J qRT-PCR was carried out to test nuclear and cytoplasmic RNA collected from equal numbers of CRC cells under each indicated condition. Data are represented by mean ± SD, n = 3, ns denotes not significant. *P < 0.05; **P < 0.01