Fig.2

CircRNF216 suppresses migration and invasion of CRC cells. A Schematic diagram showing siRNA against circRNF216 and the overexpression vector selectively targeting the backsplice site sequences. B qRT-PCR analysis was performed to evaluate the expression levels of circRNF216 and linear RNF216 after silencing with the combined siRNA pool. C The expression of circRNF216 and linear RNF216 was analyzed by qRT-PCR after circRNF216 overexpression in CRC cells. D After transfection, WB assay was conducted to evaluate N-cadherin gene expression in HCT116 and SW480 cells. E–H Transwell and wound healing assays suggested that circRNF216 suppressed cell migration and invasion. Data are represented by mean ± SD, n = 3, ns denotes not significant. *P < 0.05; **P < 0.01