Fig. 3

CircRNF216 acts as a sponge for miR-576-5p. A The target miRNAs within circRNF216 were predicted using online bioinformatic prediction tools CircInteractome and ENCORI. B, C After transfection, qRT-PCR was conducted to validate miRNAs expression in the HCT1116 and SW480 cell lines. D Relative miR-576-5p expression levels assessed by qRT-PCR in 40 paired CRC and corresponding normal tissue samples. E, F The RNA interference and overexpression efficiency were determined by qRT-PCR. G-I HCT116 cells were adopted to perform RIP assays using the AGO2 and IgG antibodies. J The relative luciferase activities were analyzed in HCT116 cells co-transfected with miR-576-5p mimics or miR-NC and luciferase reporter vectors circRNF216-WT or circRNF216-MUT. K Schematic diagram showing circRNF216-WT and circRNF216-MUT luciferase reporter vectors. Data are represented by mean ± SD, n = 3, ns denotes not significant. *P < 0.05; **P < 0.01