Fig. 4

MiR-576-5p promotes CRC cell migration and invasion in vitro by targeting ZC3H12C. A Analysis of TargetScan, miRTarBase and miRDB databases predicted the genes as a target of miR-576-5p. B, C qRT-PCR analysis of mRNA expression of miR-576-5p-related genes. D Schematic diagram showing ZC3H12C-WT and ZC3H12C-MUT luciferase reporter vectors. E Luciferase activity in HCT116 cells co-transfected with ZC3H12C-WT or ZC3H12C-MUT and miR-576-5p mimics or NC, as determined using the dual-luciferase reporter assay system. F WB assay confirmed the ZC3H12C and N-cadherin protein expression in CRC cells after transfection with miR-576-5p. G–J The effect of miR-576-5p up-regulation or down-regulation on cell migratory capability was evaluated by transwell and wound healing assays in HCT116 and SW480 cells. Data are represented by mean ± SD, n = 3, ns denotes not significant. *P < 0.05; **P < 0.01