Fig. 5

CircRNF216 suppresses CRC cell migration and invasion by relieving miR-576-5p repression of ZC3H12C expression. A qRT-PCR showed that miR-576-5p reversed the circRNF216-induced enhancement of ZC3H12C expression. B Expression of ZC3H12C and N-cadherin in CRC cells was analyzed by WB assay, with GAPDH as a control. C FISH analysis of the co-localization of circRNF216 (red) and miR-576-5p (green) in HCT116 and SW480 cells. D–G Motility ability of CRC cells co-transfected with oe-circRNF216 and miR-576-5p mimics or si-circRNF216 and miR-576-5p inhibitor was assessed by wound healing and transwell assays. Data are represented by mean ± SD, n = 3, ns denotes not significant. *P < 0.05; **P < 0.01