Fig. 6

CircRNF216 is correlated with CD8⁺ T cell infiltration via regulating ZC3H12C. A TCGA database was utilized to examine ZC3H12C expression in CRC tissues and normal tissues. B Low ZC3H12C expression was related with a poor overall survival (OS) according to the Kaplan–Meier Plot database. C GO analysis of ZC3H12C in CRC. D The correlations of immune cell infiltrations and ZC3H12C were accessed by TISIDB database. In the analysis, positive correlations were represented by the color red, while negative correlations were represented by the color blue. The intensity of the color reflected the strength of the correlation, with darker shades indicating stronger associations. E TIMER web server was utilyzed to assess the correlation of ZC3H12C and immune cells in colon adenocarcinoma and rectum adenocarcinoma. F The correlations between ZC3H12C expression and CD8A, CD8B, GZMA, GZMB, PRF1 in colon adenocarcinoma and rectum adenocarcinoma were analyzed by TIMER web server. G CircRNF216 promoted the proliferation of CD8⁺ T cells, while ZC3H12C deficiency reduced the proliferation CD8⁺ T cells in HCT116 and SW480 cells. Data are represented by mean ± SD, n = 3, ns denotes not significant. *, P < 0.05; **, P < 0.01