Fig. 7
CircRNF216 inhibited the tumor metastasis and recruited CD8+ T cells via ZC3H12C in vivo. A, B Images and metastatic nodules number of the nude mice lungs following injections of Vector and circRNF216soe in HCT116 cells. C H&E staining indicated less metastasis after circRNF216 overexpression, with arrows indicating the metastatic foci. D, E ZC3H12C and N-cadherin expression levels were observed after lentiviral transfection in HCT116 cells by WB and q-PCR assays. F Representative IHC images showing ZC3H12C and N-cadherin expression in metastatic nodules. G Schematic of the syngeneic CRC model. H, I Tumor volumes and tumor weight of mice in subcutaneous CRC model. J Flow cytometry analysis showed that circRNF216 overexpression increased the number of tumor-infiltrating CD8+ T cells, while ZC3H12C knockdown decreased tumor infiltrating CD8+ T cells in C57BL/6J mice. K circRNF216 overexpression tumors had significantly higher amounts of Granzyme B, IFN-γ, TNF-α and perforin1 than Control tumors, while knockdown of ZC3H12C rescued circRNF216 overexpression induced high soluble factors in C57BL/6J mice. Data are represented by mean ± SD, n = 3, ns denotes not significant. *P < 0.05; **P < 0.01