Fig. 2

circMAST1 suppresses tumor growth and metastasis in CCa in vitro and in vivo. A-C The proliferative abilities of SiHa and HeLa cells were measured by the colony formation (A) and CCK-8 assay (B, C) after the overexpression of circMAST1. D, E Migration and invasion assays for SiHa and HeLa cells with circMAST1 overexpression. Original magnification, ×100. F Representative images of dissected tumors from nude mice transplanted with stable circMAST1 overexpression. G The mean weight of tumors upon sacrifice in the experimental groups. H Subcutaneous tumor growth curves of mice in different treatment groups. I, J The indicated CCa cells were injected into the footpads of the nude mice, and the popliteal LNs were enucleated and analyzed after 6 weeks of injection. K, L Representative images of enucleated popliteal LNs (K) and histogram analysis of the LN volume (L) in the indicated cells. M Representative images of immunostaining of CK as indicators of LN status. CK (pan-cytokeratin) was used as the marker of tumor cells. N Quantification of metastatic area of popliteal LN metastasis in the indicated groups. ***P < 0.001. ****P < 0.0001