Fig. 3

YAP is a downstream target of circMAST1. A, B RNA-seq analysis revealed the differentially expressed genes after the overexpression of circMAST1 in SiHa cells. C, D YAP mRNA and protein expression levels were analyzed by qRT-PCR and western blotting after circMAST1 overexpression (left) or ablation (right). E Expression levels of YAP in CCa tissues in comparison with normal tissues, as determined by qRT-PCR. F Correlation analysis of mRNA expression levels between circMAST1 and YAP in CCa tissues. G Protein expression levels of YAP were analyzed by western blotting in the indicated groups. H, I Cell proliferation ability was detected by CCK-8 in the indicated groups. J–L Transwell assays were used to detect the migration and invasion capabilities of the indicated cells. Original magnification, × 100