Fig. 6

circMAST1 blocks NAT10 from binding to and ac4C modifying YAP mRNA. A, B Diagram for circMAST1 mutation plasmid construction (A, left). The interaction between circMAST1 and NAT10 in SiHa and HeLa cells shown by pull-down assay (A, right) and RIP assay (B) when transfection with the wild type or mutant circMAST1. C RIP assays were used to measure the recruitment of NAT10 protein to YAP mRNA in the indicated groups. D Ectopic expression of circMAST1 abrogated YAP mRNA ac4C modification determined by ac4C-RIP-qPCR experiment, whereas circMAST1 mutant could not show appreciate effect. E The YAP mRNA stability confirmed by the RNA decay assay in WT and Mut circMAST1 groups of SiHa and HeLa cells. F, G YAP mRNA and protein expression levels were analyzed by qRT-PCR and western blotting in the indicated groups. H The proliferative abilities of SiHa and HeLa cells were measured by the CCK-8 assay. I, J Migration and invasion assays for SiHa and HeLa cells. Original magnification, ×100. Each experiment was performed at least three times independently. **P < 0.01. ***P < 0.001. ****P < 0.0001