Fig. 2

AW1 promoted reepithelialization and granulation tissue formation to accelerate deep second-degree burn healing in mice. A Representative images of deep-second degree burns in mice under PBS, rh-bFGF (100 ng/mL), or AW1 (1, 10, and 100 nM) treatment on days 0, 4, 8, and 14. Peptide and rh-bFGF were dissolved in PBS to obtain AW1 (1, 10, and 100 nM) and rh-bFGF (100 ng/mL) solutions. Each wound was treated with vehicle (20 µL, PBS), rh-bFGF (20 µL), or different concentrations of AW1 (20 µL) twice a day from days 0–14, respectively. B–D Quantification of wound repair rate on days 4, 8, and 14. E. H&E staining of deep-second degree burns on days 4, 8, and 14. Yellow dotted lines represent areas of neoepidermis; Es, eschar; NE, neoepidermis; GT, regenerated granulation tissue; scale bar 200 μm. F–K Quantification of neoepidermis and new granulation tissue thickness in deep-second degree burns on days 4, 8, and 14. All data are expressed as mean ± SEM from six mice (n = 6); *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001 indicate statistically significant difference compared with vehicle