Fig. 6

AW1 regulated macrophage inflammation via the TLR4/NF-κB molecular axis. A Western blotting images showing inhibitory effects of AW1 (100 nM) on NF-κB signaling pathway activation in macrophages induced by LPS. TLR4-specific inhibitor significantly suppressed AW1 activity. B, C Quantification of relative expression of Iκb, P-iκb, P65, and P-P65. D Effects of AW1 (100 nM) on NF-κB signaling pathway activation in macrophages after treatment with TLR4-specific inhibitor. E, F Quantification of relative expression of Iκb, P-iκb, P65, and P-P65. All data are expressed as mean ± SEM from five independent experiments (n = 5); ^####P < 0.0001 indicates statistically significant difference compared with vehicle, *P < 0.05, ***P < 0.001, and ****P < 0.0001 indicate statistically significant difference compared with LPS