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Fig. 2 | Cellular & Molecular Biology Letters

Fig. 2

From: N6-methyladenosine-modified circ_104797 sustains cisplatin resistance in bladder cancer through acting as RNA sponges

Fig. 2

The characteristics of circ_104797. A Diagrammatic representation depicting the formation of circ_104797; B gel electrophoresis demonstrates amplification of circ_104797 using divergent primers from total cDNA, but not from genomic DNA (gDNA); C, D RT-qPCR analysis indicating the relative abundance of GAPDH mRNA and circ_104797 in T24/CDDP (C) and 5637/CDDP (D) cells post actinomycin D (Act-D) treatment at specified time intervals; E, F RT-qPCR results confirm the resistance of circ_104797 to RNase R digestion in T24/CDDP (E) and 5637/CDDP (F) cells; G subcellular localization of circ_104797 in BCa cells as determined by FISH assay; H, I RT-qPCR assessment of circ_104797, actin, and U6 distribution in the cytoplasmic and nuclear fractions of T24/CDDP (H) and 5637/CDDP (I) cells

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