Fig. 4

ALKBH5 inhibited the m⁶A modification process of circ_104797. A, B MeRIP-qPCR analyses depicted the relative enrichment of circ_104797 when immunoprecipitated with m⁶A antibody (m⁶A-IP) versus immunoglobulin G (IgG) in T24/CDDP (A) and 5637/CDDP (B) cells in both the presence and absence of ALKBH5 overexpression; C expression levels of circ_104797 were assessed using RT-qPCR following ALKBH5 overexpression; D, E examination of circ_104797 expression in T24/CDDP (D) and 5637/CDDP (E) cells post actinomycin D treatment considering the presence or absence of ALKBH5 overexpression; F a schematic representation highlights the AGACU m⁶A motif situated at the junction of exon 6 and exon 7 in circ_104797; G dual-luciferase assays revealed the expression efficiency differences between m⁶A-WT and m⁶A-Mut in BCa cells overexpressing ALKBH5; H, I RT-qPCR analyses determined the relative abundance of GAPDH mRNA and circ_104797 in T24/CDDP (H) and 5637/CDDP (I) cells post Act-D treatment, considering the overexpression status of ALKBH5