Fig. 5
Targeting m6A methylation of circ_104797 by CRISPR/dCas13b-ALKBH5 to regulate cisplatin-resistant BCa cells proliferation and apoptosis. A A diagrammatic representation delineated the m6A site locations within circ_104797 and the regions targeted by specific guide RNA, B,C In both T24/CDDP and 5637/CDDP cells, m6A modifications (B) and expression patterns (C) of circ_104797 were assessed post-transfection with dCas13b-ALKBH5, either in conjunction with control gRNA or circ_104797-specific gRNA; D,E the proliferative capacity of T24/CDDP (D) and 5637/CDDP (E) cells, post-transfection with dCas13b-ALKBH5 and either control gRNA or circ_104797-specific gRNA, was gauged using the CCK-8 assay; F,G wound healing assays were conducted on T24/CDDP and 5637/CDDP cells post-transfection with dCas13b-ALKBH5 and the respective gRNAs, with representative images (F) and subsequent quantitative evaluations (G) presented; H apoptotic rates in T24/CDDP and 5637/CDDP cells, post-transfection with dCas13b-ALKBH5 and the respective gRNAs, were determined through ELISA; I, J the tumor volume and weights of BCa cells stably transfected with dCas13b-ALKBH5 combined with gRNA control or gRNA for circ_104797