Fig. 2

High-content screening results of a target-selective library in U-2OS cells expressing the mOrange2-Peroxisomes2 marker. A The average peroxisomal signal was plotted against Hoechst staining intensity for each chemical treatment. The signal of each channel was standardized by the mean value of the control sample. Data are summarized from three biological replicates. Group A: compounds that affect peroxisomes by altering cell cycle. Group B: compounds that increase peroxisomal abundance without altering cell cycle. Group C: compounds that reduce peroxisomal abundance without altering cell cycle. B Representative results of Hoechst intensity distribution and the relative peroxisomal signals in control and compounds from each group. C Heatmap showing the relative values (normalized to controls) of cell counts, peroxisomal spot counts per cell, peroxisomal total intensity per cell, and Hoechst intensity per cell of compound treatment in group A–C. D Representative images of control and chemical treatment from each group: Panobinostat: group A; CEP-18770 and KPT-330: group B; Naftopidi and AZD6738: group C. Scale bar: 100 μm