Fig. 3
From: High-content image screening to identify chemical modulators for peroxisome and ferroptosis
Validation of screening results by immunofluorescence (IF) staining and Western blot. A IF staining of intrinsic peroxisomal marker PMP70 in HeLa and U-2OS cell lines. High-content imaging analysis was applied to quantify PMP70 staining intensity after the treatment of selective compounds (1 μM, 24 h). Dotted line represents the mean value of control samples (DMSO treatment). Data are summarized from three biological replicates. *p < 0.05; **p < 0.01; ***p < 0.001. B Western blot analysis of PEX3 protein levels after the treatment of indicated compounds (1 μM, 24 h) in HeLa and U-2OS cell lines