Fig. 4

Peroxisomal abundance and oxidative stress. A Flow cytometry analysis of DCFDA staining (ROS sensor) in U-2OS cells after treatment with compounds that promoted peroxisomal abundance. B Flow cytometry analysis of DCFDA staining (ROS sensor) in U-2OS cells after treatment with compounds that reduced peroxisomal abundance. C U-2OS cells with peroxisomal fluorescent tag were treated with 1 μM panobinostat (HDAC inhibitor), pacritinib (JAK inhibitor), and NSC697923 (E2 conjugating enzyme inhibitor), MLN2238 (proteasome inhibitor) and KPT-185 (CRM1 inhibitor) in presence or absence of 2 mM NAC. The relative levels of peroxisomal signals were analyzed by flow cytometry. N = 3 independent experiments. *p < 0.05 versus control; **p < 0.01 versus control; ***p < 0.001 versus control; ^#p < 0.05 versus treatment; ^##p < 0.01 versus treatment