Fig. 3

High IGF2BP3 expression is induced by promoter hypomethylation and copy number gain/amplification in various tumors, including bladder cancer. A Heatmap representing IGF2BP3 mRNA levels and β-value (methylation level) of 16 CpG sites on the IGF2BP3 promoter. Sorted by IGF2BP3 mRNA levels. Blue: low value, red: high value. B Heatmap displaying the Spearman correlation coefficient between IGF2BP3 mRNA levels and β-value of CpG sites on its promoter, as well as copy number of the gene across 33 cancer types in TCGA. C Scatterplot showing the positive correlation between IGF2BP3 mRNA level and the average β-value of CpG sites on the IGF2BP3 promoter in TCGA BLCA. D The UCSC Genome Browser displays the IGF2BP3 gene along with its upstream region. Within the CpG Island of the IGF2BP3 promoter region, 16 CpG sites are underlined. These CpG sites were examined using BSP (bisulfite sequencing PCR). E Methylation status of the CpG sites presented in D in RT112/84 and BFTC905 cells. Methylated CpG sites are depicted as solid circles, whereas unmethylated CpG sites are illustrated as open circles. F WB analysis of IGF2BP3 protein levels in RT112/84 and BFTC905 cells. G, H The impact of 5-aza treatment on the protein and mRNA levels of IGF2BP3 in RT112/84 cells. I Correlation analysis of IGF2BP3 expression and copy number in TCGA BLCA. J The frequency of samples exhibiting distinct copy number alterations of IGF2BP3 in TCGA BLCA. Error bars indicate the mean ± sd from three independent experiments. ***p < 0.001, **p < 0.01, *p < 0.05