Fig. 4

IGF2BP3 expression is negatively regulated by miR-320a-3p in bladder cancer. A Correlation analysis between IGF2BP3 mRNA level and miR-320a-3p level in TCGA BLCA. B Correlation analysis between IGF2BP3 mRNA level and miR-320a level in GSE40355. C Construction of a reporter plasmid with the 3′-UTR of the IGF2BP3 gene targeted by miR-320a-3p. D, E Determination of the relative luciferase activity in 293 T cells transfected with the miR-320a-3p mimic, antagomir, or negative control (NC). Vec, vector without UTR sequence. F, G The relative miR-320a-3p level in BFTC905 cells after treatment with miR-320a-3p mimic or antagomir. H–K The effect of miR-320a-3p mimic, antagomir on the IGF2BP3 mRNA and protein level in BFTC905 cells. L Comparison of miR-320a-3p levels between normal and bladder cancer tissues in GSE40355 dataset. M–O Kaplan–Meier analysis of OS, DSS, and PFI according to miR-320a-3p expression in the TCGA BLCA. Error bars indicate the mean ± sd from three independent experiments. ***p < 0.001, **p < 0.01, *p < 0.05