Fig. 3

Sponge and human R-RAS2 homologs have similar but not identical localization in membranes of MCF-7 cells. A Colocalization (yellow) of human R-RAS2 with sponge homolog EsuRRAS2L in the membranes of human breast cancer cells MCF-7. Human R-RAS2 was fluorescently labelled with GFP, and sponge EsuRRAS2L was fluorescently labelled with CHERRY (red). Hoechst was used to stain nuclei. The experiments were repeated three times in biological duplicates. Cells were analyzed by confocal microscopy. B The C-terminal hypervariable region of R-RAS subfamily members. The proline-rich region, present in HsaRRAS and HsaRRAS2 proteins, is shown in yellow, while the cysteine residue that undergoes palmitoylation is shown in orange. Lysine residues conserved between sponge R-RAS2-like and human R-RAS2 are shown in green. The CaaX box conserved between all analyzed sequences is highlighted in pink. Esu-sponge Eunapius subterraneus, Hsa-human. Scale bar—10 μm