Fig. 4

Both sponge and human R-RAS2 are localized within recycling and late endosomes. Colocalization (yellow) of sponge EsuRRAS2L or human HsaRRAS2 fluorescently labelled with GFP (green) with markers (red) for A early endosomes (EEA1), B recycling endosomes (TfR), C late endosomes (Rab7), and D lysosomes (LAMP1) in human breast cancer cells MCF-7. Hoechst was used to stain nuclei. E Quantification of colocalization between EsuRRAS2L or HsaRRAS2 with endosomal markers was done using ImageJ Coloc2 plugin and shown as Pearson’s correlation coefficient. ***p < 0.001, n = 30 cells per group from three different experiments. Cells were analyzed by confocal microscopy. Esu-sponge Eunapius subterraneus, Hsa-human. Scale bar—10 μm