Fig. 5
From: The ancestral type of the R-RAS protein has oncogenic potential
Both sponge and human R-RAS2 are localized within overexpressed early and late endosomal marker proteins. Colocalization (yellow) of cotransfected sponge EsuRRAS2L or human HsaRRAS2 fluorescently labelled with CHERRY (red) together with markers (green) for A early endosomes (Rab5), and B late endosomes (Rab7) in human breast cancer MCF-7 cells. Hoechst was used to stain nuclei. C Quantification of colocalization between EsuRRAS2L or HsaRRAS2 with endosomal markers was done using ImageJ Coloc2 plugin and shown as Pearson’s correlation coefficient. ***p < 0.001, n = 30 cells per group from three different experiments. Cells were analyzed by confocal microscopy. Esu-sponge Eunapius subterraneus, Hsa-human. Scale bar—10 μm