Fig. 3

MRE11A negatively regulates MMR activity in HeLa cells. The left pictures represent the scatter plots of cells cotransfected with GFP-heteroduplex and mCherry plasmids as described in “Materials and Methods” section. The x-axis and y-axis represent the signal intensities of GFP and mCherry, respectively. The MMR repair efficiency was calculated as the ratio of the number of GFP-positive cells to mCherry-positive cells, and the quantification results relative to siNC or empty vector controls are shown in the right graphs. A Cells were transfected with siNC or two MRE11A siRNAs followed by GFP-heteroduplex and mCherry plasmid cotransfection after 2 days. The next day, the cells were subjected to flow cytometry for GFP and mCherry signal analysis. B Cells were transfected with empty vector, MRE11A overexpression plasmid (MRE11AOE), or MRE11A + PMS2 overexpression plasmids (MRE11OE + PMS2) followed by GFP-heteroduplex and m-cherry plasmid cotransfection after 2 days. The next day, the cells were subjected to flow cytometry for GFP and mCherry signal analysis. Data are shown as mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001, using unpaired two-tailed Student’s t test