Fig. 5

MRE11A negatively regulates PMS2 levels. Representative western blotting of the indicated protein levels in whole cells (A, B) or on chromatin (C, D) with MRE11A knockdown/overexpression or expression of flag-tagged 452–634AA of MRE11A. The right graphs show the quantification of the western blotting intensities relative to siNC or empty vector controls. The changes in the mRNA levels of PMS2 after MRE11A knockdown (A) or overexpression (B) were quantified using qPCR. Representative scatter plots of flow cytometry analysis of cells expressing GFP or mCherry, reflecting MMR repair efficiencies of cells expressing 452–634AA of MRE11A with/without PMS2 overexpression (E). The MMR repair efficiency was calculated as the ratio of the number of GFP-positive cells to mCherry-positive cells, and the quantification results relative to siNC or empty vector controls are shown in the right graphs. Data shown as mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001, using unpaired two-tailed Student’s t test