Fig. 7

Specificity validation of the dual detection system H-MRC12a and optimization results for the components of the CRISPR system. a Specificity validation results for the H-MRC12a detection system against common pathogens in the female reproductive tract. After extracting nucleic acids from each pathogen, they were added to the reaction system, and fluorescence was observed immediately under UV light after a 35-min reaction. The results demonstrate no cross-reaction with the following five pathogens and the human blood genome: V1: E. coli (ATCC 25922), V2: S. aureus (ATCC 25923), V3: C. albicans (ATCC 14053), V4: T. vaginalis, V5: N. gonorrhoeae (NGO), V6: Human Blood Genome, NC negative control reaction, P positive control using HPV 18 as the DNA template. The asterisk indicates significant differences compared to P, as determined by two-tailed Student’s t-test (****P < 0.0001). b Comparison of fluorescence intensity for different concentrations of Lba Cas12a protein, ssDNA reporter, and crRNA in the CRISPR system. The optimal component concentrations were determined as follows: Lba Cas12a 250 nM, ssDNA reporter 4 μM, and crRNA 500 nM