Fig. 8

Sensitivity validation of the dual detection system H-MRC12a with a reaction time of 40 min (Multiple RPA 20 min + CRISPR 20 min). a–f Sensitivity validation results for different concentrations of plasmids for HPV types 16, 18, 31, 33, 35, and 45, respectively. The reaction results were observed immediately under UV light after completion. The asterisk indicates significant differences compared to the negative control, as determined by two-tailed Student’s t-test (*P < 0.1, ***P < 0.001, ****P < 0.0001). ns denotes no statistical significance. NC negative control reaction