Fig. 9

Comparison of experimental results between the H-MRC12a combined detection system and a commercially available QPCR genotyping kit for detecting 8 clinical HPV 16-positive samples. Clinical samples were heat-inactivated and subjected to nucleic acid extraction using the nanomagnetic bead method. The extracted nucleic acids were then incubated in the H-MRC12a combined detection system for 40 min (Multiple RPA incubation for 20 min, followed by CRISPR incubation for an additional 20 min) and immediately observed under 300 nm UV light. a Fluorescence curves and CT values obtained from the commercially available QPCR genotyping kit for the 8 clinical samples. b Real-time fluorescence signals (recorded using the Q160 LongGene portable QPCR instrument) of the H-MRC12a dual detection system for the clinical samples and negative control after 20 min of incubation in the CRISPR step. c Photographs taken under UV light of the 8 clinical samples incubated for 40 min in the H-MRC12a detection system. S16-1~S16-8: Identification numbers of the 8 HPV 16-positive clinical samples; CRH1~6: Tubes containing crRNA specific for HPV 16, 18, 31, 33, 35, and 45, respectively, for genotyping detection; NC: Negative control; PC: Positive control