A multiplex RPA-CRISPR/Cas12a-based POCT technique and its application in human papillomavirus (HPV) typing assay

Table 2 Sequences of crRNAs and the ssDNA Reporter

Oligo Name	Sequences(5′–3′)	Targeted Typing
CRH1	AAUUUCUACUGUUGUAGAUUACUGCGUGUAGUAUCAACAACAG	HPV 16
CRH2	AAUUUCUACUGUUGUAGAUCUGGCAUAAUCAAUUAUUU	HPV 18
CRH3	AAUUUCUACUGUUGUAGAUUUGGGGCAAUCAGUUAUUU	HPV 31
CRH4	AAUUUCUACUGUUGUAGAUUGCACACAAGUAACUAGUGACA	HPV 33
CRH5	AAUUUCUACUGUUGUAGAUUUACUGUAGUUGAUACAAC	HPV 35
CRH6	AAUUUCUACUGUUGUAGAUUUACUGUAGUGGACACUAC	HPV 45
ssDNA reporter	6-FAM-TTTTTTTT-BHQ1 [55]	–

ISSN: 1689-1392