Fig. 1

Loss of miR-101-3p supports the oncogenic properties of melanoma. A GSEA analysis: enrichment plot for miR-101-3p comparing a preranked gene list based on published data [29]. B Quantitative real-time PCR analysis of miRNA expression of miR-101-3p in NHEM, MBrC and primary tumor (PT) and metastasis (Met) melanoma cell lines (one-way ANOVA and subsequent Tukey’s multiple comparison test). C miRNA sequencing analysis (normalized counts) of different MM cell lines compared with NHEM and MBrCs (one-way ANOVA and subsequent Tukey’s multiple comparison test) [44]. D Clonogenic assay with MEL-JUSO, SK-MEL-28, and MV3 treated with miR-101-3p mimic (72 h) and respective siCtrl. Graphs show number of colonies and size of colonies. Representative images of colonies of MEL-JUSO, SK-MEL-28, and MV3 stained with Crystal Violet, treated with miR-101-3p mimic and siCtrl (Student’s t-test). E Real-time cell analysis in dependence on impedance measurement of MEL-JUSO, SK-MEL-28, and MV3 treated with miR-101-3p mimic (72 h) and respective siCtrl (Student’s t-test). F Counted cell number after transfection of MEL-JUSO, SK-MEL-28, and MV3 with miR-101-3p mimic (72 h) monitoring showed reduced proliferation compared with siCtrl (Student’s t-test). Bars represent mean ± SEM (*p ≤ 0.05, ns not significant)