Fig. 2

Predicted role of miR-101-3p in melanoma based on transcriptome analyses and bioinformatic analyses. A Principal component analysis of log-transformed normalized counts of the RNA-seq, showing clear differences in the gene expression of miR-101-3p mimic- and siCtrl-transfected cells. GSEA enrichment plot (B) and distribution of all significantly enriched miR-target gene sets. (C) Scatter plot of all significant normalized enrichment scores (NES with FDR < 0.25) showing the influence of miR-101-3p in regards of microRNA target genes in miR-101-3p mimic-transfected cells. D Heatmap of significantly up- (red) and downregulated (blue) miR-101-3p target genes found in miR101-3p mimic compared with siCtrl-transfected cells. E Top 10 overrepresented hallmark gene sets from MSigDB v22 of downregulated miR-101-3p target genes using EnrichR html version. F Overlap of 40 enriched miR101-3p target genes within the top 20 overrepresented gene sets determined by EnrichR analysis using clustergram [93]