Fig. 5
From: Loss of miR-101-3p in melanoma stabilizes genomic integrity, leading to cell death prevention
DNA damage induced apoptosis by PARP cleavage and TUNEL assay in miR-101-3p mimic- and siCtrl-treated cells. A–C Western blot analysis of full-length PARP and cleaved PARP following 72 h treatment with miR-101-3p mimic and siCtrl in MEL-JUSO, SK-MEL-28, and MV3 using the total of full-length PARP and cleaved PARP for calculating the ratio of cleaved PARP. Representative images of PARP western blots in MEL-JUSO, SK-MEL-28, and MV3 (Student’s t-test). D TUNEL assay for apoptosis detection by DNA strand breaks. Quantification and representative images of dUTP nick-end labeled DNA-strands (DAPI, GFP, Merge) of MEL-JUSO, SK-MEL-28, and MV3 transfected with miR-101-3p mimic (72 h) and siCtrl, respectively. Scale bars, 20 µm (Student’s t-test). Bars show mean ± SEM (*p ≤ 0.05, ns not significant)