Fig. 6

Reexpression of miR-101-3p and its influence on DNA damage and stress induction. A Western blot analysis of γH2AX following treatment 72 h with miR-101-3p mimic and siCtrl in MEL-JUSO and MV3 using β-actin as housekeeper (Student’s t-test). B Immunofluorescence staining of promyelocytic leukemia protein (PML) expression in MEL-JUSO, SK-MEL-28, and MV3 transfected with miR-101-3p mimic and siCtrl for 72 h as DNA damage detection (Student’s t-test). Representative images of PML nuclear bodies (red) and nuclear staining with DAPI (blue). Scale bars, 20 µm. C GSEA enrichment plot of Hallmark DNA_Repair (FDR < 25%), illustrating the profile of the running enrichment score (green) and positions of the enriched gene set and the rank-ordered list of genes differentially expressed in melanoma cells treated with miR-101-3p mimic and siCtrl, respectively. Genes upregulated in miR-101-3p mimic melanoma cells are shown on the left side of the graph in red, and downregulated ones on the right side in blue. Bars show mean ± SEM (*p ≤ 0.05, ns not significant)