Fig. 1
The activation of Drp1 and mitochondrial fragmentation are early events in renal IRI. A Representative images of H&E staining, KIM-1 and NGAL immunohistochemistry, Scale bar = 50 µm. B Pathological score of tubular damage, quantification of KIM-1 and NGAL positive tubules. C The serum creatinine level and BUN level. D Representative electron micrographs of mitochondrial morphology in proximal tubule cells. E Representative Immunoblot and quantitative analysis of p-Drp1(Ser616) in kidney tissue. β-actin was used as a loading control. F Representative Immunoblot and densitometry analysis of Drp1 in renal cytosolic and mitochondrial fractions. COX IV and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were used as loading controls of mitochondrial and cytosolic fractions, respectively. G Representative Immunoblot and quantitative analysis of P-Drp1(Ser616) in mPTCs cells.β-actin was used as a loading control. H Representative Immunoblot and densitometry analysis of Drp1 in mPTCs cells cytosolic and mitochondrial fractions. COX IV and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were used as loading controls of mitochondrial and cytosolic fractions, respectively. Quantitative data are expressed as mean ± SD (n = 5). *P < 0.05 versus respective Sham group or Control group