Fig. 5

P110 reduces the translocation of Drp1 to the mitochondria and mitochondrial fission. A Representative electronic microscopy images of proximal tubular cells and percentage of proximal tubule cells with mostly fragmented mitochondria (1% filamentous mitochondria). B Fluorescence after mitoRed transfection and the number of cells with fragmented mitochondria was quantified in each condition. C Representative Immunoblot and densitometry analysis of Drp1 in renal cytosolic and mitochondrial fractions. COX IV and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were used as loading controls of mitochondrial and cytosolic fractions, respectively. D Representative immunoblots densitometry analysis of Drp1 in mPTCs cells cytosolic and mitochondrial fractions. COX IV and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were used as loading controls of mitochondrial and cytosolic fractions, respectively. Quantitative data are expressed as mean ± SD. *P < 0.05 versus respective Sham/Control group; ^#P < 0.05 versus IR/ATP-DR group