Fig. 6

P110 reduces the interaction between Fis1 and Drp1 induced by ATP depletion. A–C Immunoprecipitation analysis of the Fis1-Drp1 interaction 、MFF-Drp1 interaction and the MiD51-Drp1 interaction. E Representative confocal fluorescence microscopy of Drp1 and Fis1 in mPTC cells. Scale bar = 10 μm. D mPTC cells were treated with the P110 (1 µM) for 3 h followed by treatment with ATP-depletion for 2 h. Following a brief cross-linking, cells were homogenized. Total cell lysates were then subjected to immunoprecipitation (IP) with anti-Fis1 antibodies, and the immunoprecipitates were analyzed by immunoblotting (IB) with anti-Drp1antibodies. Quantification of Drp1/Fis1 expression. Quantitative data are expressed as mean ± SD. *P < 0.05 versus respective Control group;#, P < 0.05 versus ATP-DR group