Fig. 7

P110 reduces the translocation of Bax induced by ATP depletion and the release of mtDNA. A Representative confocal fluorescence microscopy of Bax and Mitotracker in mPTC cells. Scale bar = 10 μm B–D Representative Immunoblot and densitometry analysis of Bax in renal cytosolic and mitochondrial fractions. COX IV and GAPDH were used as loading controls of mitochondrial and cytosolic fractions, respectively. E Representative confocal fluorescence microscopy of dsDNA and Fis1 in mPTC cells. Scale bar = 10 μm. F Ratio of cytoplasmic to total mtDNA determined by qPCR analysis of mPTC cells. Quantitative data are expressed as mean ± SD. *P < 0.05 versus respective Sham/Control group; ^#P < 0.05 versus IR/ATP-DR group