Fig. 8

P110 alleviates the cGAS-STING pathway induced by IRI. A, B Representative Immunoblot and quantitative analysis of cGAS,p-TBK1,p-p65 and p-IRF3 in kidney tissue. Quantitative data represent the relative ratio to total TBK1 or p65 or IRF3.Cyclophilin B (CycB) was used as loading control. C Representative images of STING immunohistochemistry. Scale bar = 50 μm. D q-PCR analysis of MCP-1, Irf7,Cxcl10,Ifit1,Ifit3,Isg15,Cgas and Ifnb1.Quantitative data are expressed as mean ± SD. *P < 0.05 versus respective Sham group; ^#P < 0.05 versus IR group