Fig. 1

Alcohol consumption increases hepatic GSTM1 expression in ALD mice. A, B GSTM1 mRNA and protein expression, and C GST activity in the Lieber-DeCarli diet plus single binge model. D, E GSTM1 mRNA and protein expression in the chronic alcohol-fed model. F–H GSTM1 protein expression in mouse AML-12 hepatocytes, primary mouse hepatocytes, and human VL-17A hepatocytes. Cells were cultured with media containing ethanol for 48 h. Protein band intensity was quantified by ImageJ. Data are presented as means ± SD. Statistical comparisons were made using one-way ANOVA with Tukey’s post hoc test or one Student’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001 versus corresponding control. PF pair-fed, AF alcohol-fed. A–E (n = 6); F–H (n = 4)